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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for anti-PDK1 antibody - OAPA00305 |
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Product Format | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
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Clonality | Monoclonal |
Clone | PAT2D6AT. |
Isotype | Mouse IgG1 heavy chain and kappa light chain. |
Host | Mouse |
Application | WB |
:: | Product Type: Mouse Anti Human Monoclonal. |
:: | Product Introduction: PDK1 takes part in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase which is part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 inhibits glioblastoma growth. PDK1 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the e1 alpha subunit, therefore contributing to the regulation of glucose metabolism. PDK1 kinase activity is negatively regulated by binding to 14-3-3. |
Reconstitution and Storage | For periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles. Stability: 12 months at -20C. 1 month at 4C. |
Immunogen | The immunogen for anti-PDK1 antibody: anti-human PDK1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PDK1 amino acids 29-436 purified from E. coli. |
Purity | PDK1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
Application Info | PDK1 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 1000. Recommended starting dilution is 1:250. |
Gene Symbol | PDK1 |
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Alias Symbols | Pyruvate dehydrogenase kinase isoform 1, PDK1, Pyruvate dehydrogenase (lipoamide) kinase isozyme 1 mitochondrial, PDK1 Antibody, Pyruvate Dehydrogenase Kinase Isozyme 1, Mouse Anti Human |
NCBI Gene Id | 5163 |
Protein Name | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial |
Description of Target | Mouse Anti Human Pyruvate Dehydrogenase Kinase Isozyme 1 |
Uniprot ID | Q15118 |
Protein Accession # | NP_002601.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PDK1 Antibody (OAPA00305)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "".
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How long will it take to receive "PDK1 Antibody (OAPA00305)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "PDK1 Antibody (OAPA00305)" provided in?
This item is provided in "1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PDK1 Antibody (OAPA00305)"?
This target may also be called "Pyruvate dehydrogenase kinase isoform 1, PDK1, Pyruvate dehydrogenase (lipoamide) kinase isozyme 1 mitochondrial, PDK1 Antibody, Pyruvate Dehydrogenase Kinase Isozyme 1, Mouse Anti Human" in publications.
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What is the shipping cost for "PDK1 Antibody (OAPA00305)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PDK1 Antibody (OAPA00305)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PDK1 Antibody (OAPA00305)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PDK1 Antibody (OAPA00305)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PDK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PDK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PDK1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PDK1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PDK1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PDK1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.