Catalog No: OKCD02791 (Formerly GWB-TS9M27)
Size:96WELLS
Price: $700.00
SKU
OKCD02791
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Datasheets/ManualsPrintable datasheet for PDIA4 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)96-103%82-96%79-101%80-89%
EDTA Plasma (n=5)79-101%91-105%90-98%80-97%
Heparin Plasma (n=5)85-92%97-104%98-105%91-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A4 (PDIA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A4 (PDIA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A4 (PDIA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Disulfide Isomerase A4 (PDIA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-8881
EDTA Plasma (n=5)78-10489
Heparin Plasma(n=5)80-10182
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A4 (PDIA4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A4 (PDIA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PDIA4 Microplate96 Wells (12 x 8 Well strips)
PDIA4 Lyophilized Standard2
100X Biotinylated PDIA4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.112 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A4 (PDIA4). No significant cross-reactivity or interference between Protein Disulfide Isomerase A4 (PDIA4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPDIA4
Gene Full Nameprotein disulfide isomerase associated 4
Alias SymbolsC, Cai, ERp, Erp72, ERp-72, AI987846
NCBI Gene Id12304
Protein NameProtein disulfide-isomerase A4
Uniprot IDP08003
Protein Accession #NP_033917.2
Nucleotide Accession #NM_009787.2
Protein Size (# AA)638
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