Catalog No: OKEH06342
Size:96T
Price: $625.00
SKU
OKEH06342
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Pdia3 ELISA Kit (Mouse) (OKEH06342) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pdia3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pdia3 and incubated. The Pdia3 found in the sample or standards competes with the biotinylated Pdia3 for limited binding sites on the immobilized anti-Pdia3 antibody. Excess unbound biotinylated Pdia3 and sample or standard Pdia3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pdia3 captured in the well and inversely proportional to the amount of Pdia3 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 87.4% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <= 6.9% (n = 20) Mean Inter-Assay Precision - <= 9.4% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.78 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Mouse Protein disulfide-isomerase A3 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Pdia3 |
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Gene Full Name | protein disulfide isomerase associated 3 |
Alias Symbols | 58 kDa glucose-regulated protein;58 kDa microsomal protein;58kDa;disulfide isomerase ER-60;E;endoplasmic reticulum protein;endoplasmic reticulum resident protein 57;endoplasmic reticulum resident protein 60;ER protein 57;ER protein 60;Erp;ERp57;ERp60;ERp61;glucose regulated protein, 58 kDa;Grp;Grp58;P;p58;PDI;PDI-;PDI-Q2;phospholipase C, alpha;PI-PLC;PLC[a];Plca;protein disulfide-isomerase A3. |
NCBI Gene Id | 14827 |
Protein Name | P27773 |
Description of Target | Protein disulfide-isomerase A3 |
Uniprot ID | https://www.uniprot.org/uniprot/P27773 |
Protein Accession # | NP_033882 |
Nucleotide Accession # | NM_009752 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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