PDI Protein (OOPA00159)

Catalog No: OOPA00159 (Formerly GWB-2D13C5)

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: Escherichia Coli.
• Additional Information: Solubility: It is recommended to Reconstitution: Reconstitute the lyophilized PDI in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
  Product Introduction: Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). They act also as chaperones, and are, therefore, part of a quality-control system for the correct folding of the proteins in the same subcellular compartment. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.
  Product Description: PDI Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids and having a molecular mass of 62.4 kDa. The PDI is fused to a 12 amino acid His tag (515 a.a. total) at N-terminal and purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Protein Disulfide Isomerase although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Human PDI should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
• Formulation: The PDI protein (1mg/ml)solution was lyophilized from PBS pH-7.
• Purification: Greater than 95.0% as determined by a) Analysis by RP-HPLC. b) Analysis by SDS-PAGE.
• Purity: Greater than 95.0% as determined by a) Analysis by RP-HPLC. b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.

Target Info

• Gene Symbol: PDI
• Alias Symbols: Protein Disulfide Isomerase, PDI, EC 5.3.4.1, Prolyl 4-hydroxylase subunit beta, Cellular thyroid hormone-binding protein, p55, P4HB, ERBA2L, PDIA1, PO4DB, DSI, GIT, PHDB, PO4HB, PROHB, P4Hbeta.
• Protein Size (# AA): Recombinant