Catalog No: OPCD06073
Price: $0.00
SKU
OPCD06073
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for PDHA1 Recombinant Protein (OPCD06073) |
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Predicted Species Reactivity | Homo sapiens|Human |
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Product Format | Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. |
Host | E.coli |
Conjugation | Unconjugated |
Application | Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot |
Additional Information | Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method) Residues: Phe30-Ser390 Subcellular Location: Mitochondrion Isoelectric Point: 6.5 |
:: | Phenomenon Explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice Variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affect the charge of the protein. 3. Post-translational modificaiton: Phosphorylation, glycosylation, methylation, etc. 4. Post-translational cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. |
Reconstitution and Storage | 2°C to 8°C|-80°C |
Concentration | 1000 ug/mL (prior to lyoph) |
Purity | > 90% |
Source | E.coli |
Tag | N-terminal His Tag |
Gene Symbol | PDHA1 |
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Gene Full Name | pyruvate dehydrogenase E1 subunit alpha 1 |
Alias Symbols | PDHA;PDHAD;PDHCE1A;PDHE1-A type I;PHE1A;pyruvate dehydrogenase (lipoamide) alpha 1;pyruvate dehydrogenase alpha 1;pyruvate dehydrogenase complex, E1-alpha polypeptide 1;pyruvate dehydrogenase E1 alpha 1 subunit;pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial. |
NCBI Gene Id | 5160 |
Protein Name | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial |
Description of Target | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
Uniprot ID | P08559 |
Protein Accession # | NP_000275.1 |
Nucleotide Accession # | NM_000284.3 |
Protein Size (# AA) | Phe30~Ser390 |
Molecular Weight | 46kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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