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Catalog No: OPCD06073
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OPCD06073
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Datasheets/ManualsPrintable datasheet for PDHA1 Recombinant Protein (OPCD06073)
Product Info
Predicted Species ReactivityHomo sapiens|Human
Product FormatFreeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
HostE.coli
ConjugationUnconjugated
ApplicationPositive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
Additional InformationEndotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
Residues: Phe30-Ser390
Subcellular Location: Mitochondrion
Isoelectric Point: 6.5
::Phenomenon Explanation: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice Variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affect the charge of the protein.
3. Post-translational modificaiton: Phosphorylation, glycosylation, methylation, etc.
4. Post-translational cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Reconstitution and Storage2°C to 8°C|-80°C
Concentration1000 ug/mL (prior to lyoph)
Purity> 90%
SourceE.coli
TagN-terminal His Tag
Gene SymbolPDHA1
Gene Full Namepyruvate dehydrogenase E1 subunit alpha 1
Alias SymbolsPDHA;PDHAD;PDHCE1A;PDHE1-A type I;PHE1A;pyruvate dehydrogenase (lipoamide) alpha 1;pyruvate dehydrogenase alpha 1;pyruvate dehydrogenase complex, E1-alpha polypeptide 1;pyruvate dehydrogenase E1 alpha 1 subunit;pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial.
NCBI Gene Id5160
Protein NamePyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Description of TargetThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Uniprot IDP08559
Protein Accession #NP_000275.1
Nucleotide Accession #NM_000284.3
Protein Size (# AA)Phe30~Ser390
Molecular Weight46kDa
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