PDHA1 Antibody (OAAB21682)

Catalog No: OAAB21682

Size:100ul

Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293, 1299, mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (OAAB21682) (1:1,000 diluted). Predicted band size: 43KDa. Observed band size: 43KDa.

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (OAAB21682) (dilution 1:100).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC
• Additional Information: Function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Application Info: WB: 1:1000
  ICC: 1:100
• Reference: Koike K.,et al.Gene 93:307-311(1990).
  Ho L.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:5330-5334(1989).
  Huh T.L.,et al.Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases.
  Dahl H.-H.M.,et al.J. Biol. Chem. 262:7398-7403(1987).
  Maragos C.,et al.J. Biol. Chem. 264:12294-12298(1989).
  

Target Info

• Gene Symbol: PDHA1
• Gene Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Alias Symbols: PDHA, PDHAD, PHE1A, PDHCE1A
• NCBI Gene Id: 5160
• Description of Target: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P08559
• Protein Accession #: NP_000275.1
• Nucleotide Accession #: NM_000284.3
• Molecular Weight: 43 kDa