PDGFRL Chemi-Luminescent ELISA Kit (Mouse) (OKCD03871)

Catalog No: OKCD03871

Size:96WELLS

Mouse PDGFRL ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-101%	95-102%	94-103%	90-99%
  EDTA Plasma (n=5)	89-102%	88-95%	87-102%	78-101%
  Heparin Plasma (n=5)	78-91%	86-99%	84-92%	96-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) level in the sample or standard.
• ELISA Kit Range: 6.86-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-104	101
  EDTA Plasma (n=5)	93-105	96
  Heparin Plasma (n=5)	81-95	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Pdgfrl Microplate: 96 Wells (12 x 8 Well strips)
  • Pdgfrl Lyophilized Standard: 2
  • 100X Pdgfrl Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Pdgfrl
• Gene Full Name: platelet-derived growth factor receptor-like
• Alias Symbols: 1110039P19Rik;AV013190;PDGFR-like protein;platelet-derived growth factor receptor-like protein.
• NCBI Gene Id: 68797
• Protein Name: Platelet-derived growth factor receptor-like protein
• Description of Target: Platelet-derived growth factor receptor-like protein
• Uniprot ID: Q6PE55
• Protein Accession #: NP_081116
• Nucleotide Accession #: NM_026840