PDGFRA Antibody - C-terminal region (Phospho-E1063) (OAAB17179)

Catalog No: OAAB17179

Size:400ul

Western blot analysis of PDGFRA Antibody (C-term E1063) (Cat.# OAAB17179) in K562 cell line lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.

There is BioGPS gene expression data showing that PDGFRA is expressed in K562

Western blot analysis of PDGFRA antibody (C-term E1063) (Cat.# OAAB17179) in mouse lung tissue lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

PDGFRA Antibody (C-term E1063) (Cat. # OAAB17179) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
  Cellular Location: Cell membrane; Single-pass type I membrane protein. Note=The activated receptor is rapidly internalized and degraded
  Tissue Location: Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Chicken, Rat
• Concentration: 1 mg/ml
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that PDGFRA is expressed in K562

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50

Target Info

• Gene Symbol: PDGFRA
• Alias Symbols: CD140A, PDGFR2, PDGFR-2
• NCBI Gene Id: 5156
• Protein Name: Platelet-derived growth factor receptor alpha
• Description of Target: PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
• Uniprot ID: P16234
• Protein Accession #: NP_006197.1
• Molecular Weight: 122670 Da