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Catalog No: OCOA07236
Size:20UG
Price: $179.00
SKU
OCOA07236
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PDGFB Protein Lysate (OCOA07236)

Datasheets/ManualsPrintable datasheet for PDGFB Protein Lysate (OCOA07236)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionPDGFB HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolPDGFB
Gene Full Nameplatelet derived growth factor subunit B
Alias Symbolsbecaplermin;c-sis;epididymis secretory sperm binding protein;IBGC5;PDGF subunit B;PDGF, B chain;PDGF2;PDGF-2;platelet-derived growth factor 2;platelet-derived growth factor B chain;Platelet-derived growth factor beta polypeptide;platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog);platelet-derived growth factor subunit B;platelet-derived growth factor, beta polypeptide (oncogene SIS);proto-oncogene c-Sis;SIS;SSV.
NCBI Gene Id5155
Protein NameNM_033016, NP_148937, P01127
Description of TargetHomo sapiens platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 2, mRNA, Platelet-derived growth factor subunit B, platelet-derived growth factor subunit B isoform 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P01127
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_148937
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_033016
Molecular Weight25.3 kDa
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