Catalog No: OKEH02861
Price: $425.00
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Pdgfb ELISA Kit (Mouse) : 96 Wells (OKEH02861)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Pdgfb ELISA Kit (Mouse) (OKEH02861) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdgfb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pdgfb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdgfb captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
Pdgfb Microplate96 Wells (12 x 8 Well strips)
Pdgfb Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Pdgfb Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity15.62 pg/mL
SpecificityNatural and recombinant Mouse Platelet-derived growth factor subunit B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPdgfb
Gene Full Nameplatelet derived growth factor, B polypeptide
Alias Symbolsc-sis;PDGF-;PDGF subunit B;PDGF-2;PDGF-B;platelet-derived growth factor B chain;platelet-derived growth factor beta polypeptide;platelet-derived growth factor subunit B;proto-oncogene c-Sis;Sis.
NCBI Gene Id18591
Protein NameP31240
Description of TargetPlatelet-derived growth factor subunit B
Uniprot ID
Protein Accession #NP_035187.2
Nucleotide Accession #NM_011057.3
Protein Size (# AA)241
Molecular Weight27 kDa
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