Pdgfb ELISA Kit (Mouse) : 96 Wells (OKEH02861)

Catalog No: OKEH02861

Size:96T

Mouse Pdgfb ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Pdgfb ELISA Kit (Mouse) (OKEH02861) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdgfb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pdgfb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdgfb captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.5% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • Pdgfb Microplate: 96 Wells (12 x 8 Well strips)
  • Pdgfb Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Pdgfb Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 15.62 pg/mL
• Specificity: Natural and recombinant Mouse Platelet-derived growth factor subunit B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Pdgfb
• Gene Full Name: platelet derived growth factor, B polypeptide
• Alias Symbols: c-sis;PDGF-;PDGF subunit B;PDGF-2;PDGF-B;platelet-derived growth factor B chain;platelet-derived growth factor beta polypeptide;platelet-derived growth factor subunit B;proto-oncogene c-Sis;Sis.
• NCBI Gene Id: 18591
• Protein Name: P31240
• Description of Target: Platelet-derived growth factor subunit B
• Uniprot ID: https://www.uniprot.org/uniprot/P31240
• Protein Accession #: NP_035187.2
• Nucleotide Accession #: NM_011057.3
• Protein Size (# AA): 241
• Molecular Weight: 27 kDa