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Catalog No: OKCD03864
Size:96WELLS
Price: $925.00
SKU
OKCD03864
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PDGFB Chemi-Luminescent ELISA Kit (Pig) (OKCD03864)

Datasheets/ManualsPrintable datasheet for PDGFB Chemi-Luminescent ELISA Kit (Pig) (OKCD03864)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
sodium citrate plasma(n=5)96-105%87-99%89-105%86-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor Subunit B (PDGFB) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
sodium citrate plasma(n=5)98-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Pdgfb Microplate96 Wells (12 x 8 Well strips)
Pdgfb Lyophilized Standard2
100X Pdgfb Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypePlasma
Sensitivity31.2 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPDGFB
Alias SymbolsPDGF-2;Platelet-derived growth factor B chain;Platelet-derived growth factor beta polypeptide.
Protein NamePlatelet-derived growth factor subunit B
Description of TargetPlatelet-derived growth factor subunit B
Uniprot IDP20034
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