Catalog No: OKCD03863
Size:96WELLS
Price: $800.00
SKU
OKCD03863
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PDGFB Chemi-Luminescent ELISA Kit (Human) (OKCD03863)

Datasheets/ManualsPrintable datasheet for PDGFB Chemi-Luminescent ELISA Kit (Human) (OKCD03863)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
sodium citrate plasma(n=5)98-105%79-89%78-90%78-97%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor Subunit B (PDGFB) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
sodium citrate plasma(n=5)79-9981
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PDGFB Microplate96 Wells (12 x 8 Well strips)
PDGFB Lyophilized Standard2
100X PDGFB Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypePlasma
Sensitivity31.2 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPDGFB
Gene Full Nameplatelet derived growth factor subunit B
Alias Symbolsbecaplermin;c-sis;epididymis secretory sperm binding protein;IBGC5;PDGF subunit B;PDGF, B chain;PDGF2;PDGF-2;platelet-derived growth factor 2;platelet-derived growth factor B chain;Platelet-derived growth factor beta polypeptide;platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog);platelet-derived growth factor subunit B;platelet-derived growth factor, beta polypeptide (oncogene SIS);proto-oncogene c-Sis;SIS;SSV.
NCBI Gene Id5155
Protein NamePlatelet-derived growth factor subunit B
Description of TargetGrowth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
Uniprot IDP01127
Protein Accession #NP_002599
Nucleotide Accession #NM_002608
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