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Datasheets/Manuals | Printable datasheet for anti-PDGFB (ARP58509_P050-FITC) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Sheep |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | FITC: Fluorescein Isothiocyanate |
Application | WB, IHC |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human PDGFB |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Sheep: 100% |
Peptide Sequence | Synthetic peptide located within the following region: NRCWALFLSLCCYLRLVSAEGDPIPEELYEMLSDHSIRSFDDLQRLLHGD |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-PDGFB (ARP58509_P050-FITC) antibody is Catalog # AAP58509 (Previous Catalog # AAPP34718) |
Sample Type Confirmation | There is BioGPS gene expression data showing that PDGFB is expressed in HEK293T |
Subunit | B |
Reference | Chadjichristos,C.E., (2008) Circ. Res. 102 (6), 653-660 |
Gene Symbol | PDGFB |
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Gene Full Name | Platelet-derived growth factor beta polypeptide |
Alias Symbols | SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2 |
NCBI Gene Id | 5155 |
Protein Name | Platelet-derived growth factor subunit B |
Description of Target | PDGFB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor.The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. |
Uniprot ID | P01127 |
Protein Accession # | NP_002599 |
Nucleotide Accession # | NM_002608 |
Protein Size (# AA) | 241 |
Molecular Weight | 27kDa |
Protein Interactions | COL6A1; COL5A1; COL4A1; COL3A1; COL2A1; COL1A2; COL1A1; LYVE1; NRP1; ADIPOQ; MDFI; PDAP1; ART1; PDGFB; HRAS; THBS1; PDGFRA; PDGFRB; HSPG2; LRP1; A2M; SPARC; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PDGFB Antibody - N-terminal region : FITC (ARP58509_P050-FITC)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Sheep".
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How long will it take to receive "PDGFB Antibody - N-terminal region : FITC (ARP58509_P050-FITC)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "PDGFB Antibody - N-terminal region : FITC (ARP58509_P050-FITC)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PDGFB Antibody - N-terminal region : FITC (ARP58509_P050-FITC)"?
This target may also be called "SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2" in publications.
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What is the shipping cost for "PDGFB Antibody - N-terminal region : FITC (ARP58509_P050-FITC)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PDGFB Antibody - N-terminal region : FITC (ARP58509_P050-FITC)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PDGFB Antibody - N-terminal region : FITC (ARP58509_P050-FITC)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "27kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PDGFB Antibody - N-terminal region : FITC (ARP58509_P050-FITC)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PDGFB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PDGFB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PDGFB"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PDGFB"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PDGFB"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PDGFB"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.