PDGFA ELISA Kit (Bovine) (OKEH07302)

Catalog No: OKEH07302

Size:96T

Bovine PDGFA ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology PDGFA ELISA Kit (Bovine) (OKEH07302) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDGFA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDGFA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDGFA captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.0% (n = 20)
  Mean Inter-assay CV%: <=8.2% (n = 20)
• ELISA Kit Component:
  • PDGFA Microplate: 96 Wells (12 x 8 Well strips)
  • PDGFA Lyophilized Standard: 2
  • 100X Biotinylated PDGFA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.
  Subcellular Location: Secreted Released by platelets upon wounding.
  Unigene: Bt.18780
  SMR: Q2KJ15
  String: 9913.ENSBTAP00000019323
  KEGG: bta:505908
  Pfam: PF00341
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39pg/mL
• Specificity: Natural and recombinant Bovine Platelet-derived growth factor subunit A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PDGFA
• Gene Full Name: platelet derived growth factor subunit A
• Alias Symbols: PDGF subunit A;platelet-derived growth factor A chain;platelet-derived growth factor alpha polypeptide;platelet-derived growth factor subunit A.
• NCBI Gene Id: 505908
• Protein Name: Q2KJ15
• Description of Target: Platelet-derived growth factor subunit A
• Uniprot ID: https://www.uniprot.org/uniprot/Q2KJ15
• Protein Accession #: NP_001068699.1
• Nucleotide Accession #: NM_001075231.1