PDGFA Antibody (OASA08082)
|Predicted Species Reactivity||Human|
|Product Format||Purified IgG - lyophilised|
|::||Preservative Stabilisers: None present|
Antiserum Preparation: Antisera to human PDGF-AA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
|::||Approx Protein Conc: IgG concentration 1.0mg/ml |
Buffer Solutions: Phosphate buffered saline pH7.4
|Reconstitution and Storage||Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.|
Storage: Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Replacement Item||This antibody may replace item sc-122461 from Santa Cruz Biotechnology.|
|Immunogen||The immunogen for anti-PDGFA antibody: recombinant human PDGF-AA.|
|Predicted Homology Based on Immunogen Sequence||Human|
|Datasheets/Manuals||Printable datasheet for anti-PDGFA antibody - OASA08082|
|Application Info||ELISA: 0.5ug/ml|
Western Blotting: 0.1 - 0.2ug/ml
Functional Assays: 0.17 - 0.19ug/ml
|Target Reference||1. Rorsman, F. et al. (1998) Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: Alternative exon usage predicts two different precursor proteins. Mol. Cell. Biol. 8: 571-577.|
2. Ingram, J.L. et al. (2004) IL-13 and IL-1beta promote lung fibroblast growth through coordinated up-regulation of PDGF-AA and PDGF-Ralpha. FASEB J. 18: 1132-1134.
|Alias Symbols||PDGF1, PDGF-A, PDGFA|
|NCBI Gene Id||5154|
|Protein Name||Platelet-derived growth factor subunit A|
|Description of Target||RABBIT ANTI HUMAN PDGF AA HOMODIMER|
|Protein Accession #||NP_002598.4|
|Protein Size (# AA)||211|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express PDGFA.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express PDGFA.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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