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PDGFA Antibody (OASA08082)

0.1 mg
$609.00
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Gene Symbol:
PDGFA
NCBI Gene Id:
5154
Protein Name:
Platelet-derived growth factor subunit A
Swissprot Id:
P04085
Protein Accession #:
NP_002598.4
Alias Symbols:
PDGF1, PDGF-A, PDGFA
Replacement Item:
This antibody may replace item sc-122461 from Santa Cruz Biotechnology.
Description of Target:
RABBIT ANTI HUMAN PDGF AA HOMODIMER
Protein Size (# AA):
211
Host:
Rabbit
Clonality:
Polyclonal
Application:
ELISA, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PDGFA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PDGFA.
Immunogen:
The immunogen for anti-PDGFA antibody: recombinant human PDGF-AA.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - lyophilised
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
Storage: Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-PDGFA antibody - OASA08082
Specificity:
PDGF
Isotype:
Polyclonal IgG
Application Info:
ELISA:    0.5ug/ml
Western Blotting:    0.1 - 0.2ug/ml
Functional Assays:    0.17 - 0.19ug/ml
:::
Preservative Stabilisers: None present
Antiserum Preparation: Antisera to human PDGF-AA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Rorsman, F. et al. (1998) Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: Alternative exon usage predicts two different precursor proteins. Mol. Cell. Biol. 8: 571-577.
2. Ingram, J.L. et al. (2004) IL-13 and IL-1beta promote lung fibroblast growth through coordinated up-regulation of PDGF-AA and PDGF-Ralpha. FASEB J. 18: 1132-1134.

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