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Catalog No: OKEH03117
Size:96T
Price: $525.00
SKU
OKEH03117
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PDE5A ELISA Kit (Rat) (OKEH03117) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pde5a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Pde5a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pde5a captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay Precision - ≤ 6.1%, n = 20
Mean Inter-Assay Precision - ≤ 8.9%, n = 20
ELISA Kit Component
ComponentAmount
Anti-Pde5a Microplate96 Wells (12 x 8 Well strips)
Pde5a Lyophilized Standard2
100X Biotinylated Pde5a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Rat cGMP-specific 3',5'-cyclic phosphodiesterase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPde5a
Gene Full Namephosphodiesterase 5A
Alias SymbolsCGB-PDE;cGMP-binding cGMP specific phosphodiesterase 5A2;cGMP-binding cGMP-specific phosphodiesterase;cGMP-specific 3',5'-cyclic phosphodiesterase;PDE5A2;phosphodiesterase 5A, cGMP-specific;phosphodiesterase type 5.
NCBI Gene Id171115
Protein NameO54735
Description of TargetcGMP-specific 3',5'-cyclic phosphodiesterase
Uniprot IDhttps://www.uniprot.org/uniprot/O54735
Protein Accession #NP_598268.1
Nucleotide Accession #NM_133584.1
Protein Size (# AA)833
Molecular Weight95 kDa
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