PDE3A ELISA Kit (Rat) (OKCD01263)

Catalog No: OKCD01263 (Formerly GWB-KBBMT7)

Size:96WELLS

Rat Pde3a ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 3A, cGMP Inhibited (PDE3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 3A, cGMP Inhibited (PDE3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 3A, cGMP Inhibited (PDE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphodiesterase 3A, cGMP Inhibited (PDE3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.781-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphodiesterase 3A, cGMP Inhibited (PDE3A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphodiesterase 3A, cGMP Inhibited (PDE3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Pde3a Microplate: 96 Wells (12 x 8 Well Strips)
  • Pde3a Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated Pde3a Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.27 ng/mL.
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 3A, cGMP Inhibited (PDE3A). No significant cross-reactivity or interference between Phosphodiesterase 3A, cGMP Inhibited (PDE3A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Pde3a
• Gene Full Name: phosphodiesterase 3A
• Alias Symbols: CGI-PDE A;cGMP-inhibited 3',5'-cyclic phosphodiesterase A;cyclic GMP-inhibited phosphodiesterase A;cyclic nucleotide phosphodiesterase;phosphodiesterase 3A, cGMP inhibited;RNPDE3A.
• NCBI Gene Id: 50678
• Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• Description of Target: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
• Uniprot ID: Q62865
• Protein Accession #: NP_059033
• Nucleotide Accession #: NM_017337.1
• Protein Size (# AA): 1141