Catalog No: OKEH02197
Size:96T
Price: $525.00
SKU
OKEH02197
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PDE1C ELISA Kit (Human) (OKEH02197) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDE1C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDE1C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDE1C captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
PDE1C Microplate96 Wells (12 x 8 Well strips)
PDE1C Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PDE1C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.15 ng/mL
SpecificityNatural and recombinant Human Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPDE1C
Gene Full Namephosphodiesterase 1C
Alias Symbols3',5'-cyclic-AMP phosphodiesterase;3',5'-cyclic-GMP phosphodiesterase;calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C;cam-PDE 1C;DFNA74;hCam-3;Hcam3;Human 3',5' cyclic nucleotide phosphodiesterase (HSPDE1C1A);phosphodiesterase 1C, calmodulin-dependent 70kDa.
NCBI Gene Id5137
Protein NameQ14123
Description of TargetCalcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
Uniprot IDhttps://www.uniprot.org/uniprot/Q14123
Protein Accession #NP_001177985.1
Nucleotide Accession #NM_001191056.2
Protein Size (# AA)709
Molecular Weight81 kDa
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