PDCD4 Antibody (OACA07074)

Catalog No: OACA07074

Immunoprecipitating PDCD4 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of OACA09842 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/5,0000)
Lane 2: OACA09842(4ug)+ Hela whole cell lysate(500ug)
Lane 3: Hela whole cell lysate (20ug)

Western blot
All lanes: Programmed cell death protein 4 antibody at 5ug/ml
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 52,51 kDa
Observed band size: 52 kDa

Immunoprecipitating PDCD4 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of OACA09842 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/5,0000)
Lane 2: OACA09842(4ug)+ Hela whole cell lysate(500ug)
Lane 3: Hela whole cell lysate (20ug)

Western blot
All lanes: Programmed cell death protein 4 antibody at 5ug/ml
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 52,51 kDa
Observed band size: 52 kDa

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunoprecipitation|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Programmed cell death protein 4 protein (1-260AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E.Science 314:268-274(2006). [2]"Structural basis for translational inhibition by the tumour suppressor Pdcd4." Loh P.G., Yang H.S., Walsh M.A., Wang Q., Wang X., Cheng Z., Liu D., Song H. EMBO J. 28:274-285(2009). [3]"PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains." Suzuki C., Garces R.G., Edmonds K.A., Hiller S., Hyberts S.G., Marintchev A., Wagner G. Proc. Natl. Acad. Sci. U.S.A. 105:3274-3279(2008).

Target Info

• Gene Symbol: PDCD4
• Gene Full Name: programmed cell death 4
• Alias Symbols: H731;neoplastic transformation inhibitor protein;nuclear antigen H731;Nuclear antigen H731-like;programmed cell death 4 (neoplastic transformation inhibitor);programmed cell death protein 4;protein 197/15a.
• NCBI Gene Id: 27250
• Protein Name: Programmed cell death protein 4
• Description of Target: Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).
• Uniprot ID: Q53EL6
• Protein Accession #: NP_055271
• Nucleotide Accession #: NM_014456