Catalog No: OKCD06760
Size:96WELLS
Price: $600.00
SKU
OKCD06760
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Datasheets/ManualsPrintable datasheet for PDCD1 ELISA Kit (Human) (OKCD06760)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 (PD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 (PD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 1 (PD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Programmed Cell Death Protein 1 (PD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 1 (PD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 1 (PD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PDCD1 Microplate96 Wells (12 x 8 Well strips)
PDCD1 Lyophilized Standard2
100X PDCD1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.063ng/mL
Gene SymbolPDCD1
Gene Full Nameprogrammed cell death 1
Alias SymbolsCD279;hPD-1;hPD-l;hSLE1;PD1;PD-1;programmed cell death 1 protein;programmed cell death protein 1;protein PD-1;SLEB2;systemic lupus erythematosus susceptibility 2.
NCBI Gene Id5133
Protein NameProgrammed cell death protein 1
Description of TargetInhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.
Uniprot IDQ15116
Protein Accession #NP_005009.2
Nucleotide Accession #NM_005018.2
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