PDCD1 Antibody : PE (OATA00389)

Catalog No: OATA00389

C57Bl/6 splenocytes were stimulated with ConA and then stained with APC Anti-Mouse CD3e (OATA00010) and 0.5 ug PE Anti-Mouse CD279 (PD-1) (OATA00389) (right panel) or 0.5 ug PE Armenian hamster IgG isotype control (left panel).

Product Info

• Tested Species Reactivity: Mouse
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: J43.1
• Isotype: Armenian Hamster IgG
• Host: Hamster
• Conjugation: PE: Phycoerythrin
• Application: FC
  Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
  Emmision: 578 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD279 (PD-1)
• Application Info: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
• Reference:

  Hams E, McCarron MJ, Amu S, Yagita H, Azuma M, Chen L, and Fallon PG. 2011. J. Immunol. 186:5648-5655. (in vivo blocking)

  Rivas MN, Weatherly K, Hazzan M, Vokaer B, Dremier S, Gaudray F, Goldman M, Salmon I, and Braun MY. 2009. 183:4284-4291. (in vitro blocking)

  Koehn BH, Ford ML, Ferrer IR, Borom K, Gangappa S, Kirk AD, and Larsen CP. 2008. J. Immunol. 181:5313-5322. (in vivo blocking)

  Brooks DG, Ha S-J, Elsaesser H, Sharpe AH, Freeman GJ, and Oldstone MBA. 2008. Proc. Natl. Acad. Sci. 105:20428-20433. (Flow cytometry)

  Ansari MJI, Salama AD, Chitnis T, Smith RN, Yagita H, Akiba H, Yamazaki T, Azuma M, Isai H, Khoury SJ, Auchincloss H, and Sayegh MH. 2003. J. Exp. Med. 198:63-71. (Immunohistochemistry - frozen tissue, in vivo blocking)

  Agata Y, Kawasaki A, Nishimura H, Ishida Y, Tsubat T, Yagita H, and Honjo T. 1996. Int. Immunol. 8:765-772. (Immunoprecipitation)

Target Info

• Gene Symbol: PDCD1
• Gene Full Name: programmed cell death 1
• Alias Symbols: PD-, Pdc, PD-1, Pdc1, Ly101
• NCBI Gene Id: 18566
• Protein Name: programmed cell death protein 1