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Catalog No: OKCD02785
Size:96WELLS
Price: $650.00
SKU
OKCD02785
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Datasheets/ManualsPrintable datasheet for PCOLCE ELISA Kit (Mouse) (OKCD02785)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)78-91%97-104%84-104%88-101%
EDTA Plasma (n=5)86-94%87-97%80-89%99-105%
Heparin Plasma (n=5)96-104%83-97%86-93%85-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen C-Endopeptidase Enhancer (PCOLCE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen C-Endopeptidase Enhancer (PCOLCE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen C-Endopeptidase Enhancer (PCOLCE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Procollagen C-Endopeptidase Enhancer (PCOLCE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)86-10193
EDTA Plasma (n=5)90-9793
Heparin Plasma(n=5)86-10191
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen C-Endopeptidase Enhancer (PCOLCE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen C-Endopeptidase Enhancer (PCOLCE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PCOLCE Microplate96 Wells (12 x 8 Well strips)
PCOLCE Lyophilized Standard2
100X Biotinylated PCOLCE Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.228 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Procollagen C Proteinase Enhancer 1 (PCPE1). No significant cross-reactivity or interference between Procollagen C Proteinase Enhancer 1 (PCPE1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPcolce
Gene Full Nameprocollagen C-endopeptidase enhancer protein
Alias SymbolsAst;astroglial cell transcript 2;Astt;Astt2;Astt-2;P14;PCPE-1;procollagen C-endopeptidase enhancer 1;procollagen COOH-terminal proteinase enhancer 1;procollagen C-proteinase enhancer 1;procollagen C-proteinase enhancer protein;type 1 procollagen C-proteinase enhancer protein;type I procollagen COOH-terminal proteinase enhancer.
NCBI Gene Id18542
Protein NameProcollagen C-endopeptidase enhancer 1
Description of TargetBinds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.
Uniprot IDQ61398
Protein Accession #NP_032814.2
Nucleotide Accession #NM_008788.2
Protein Size (# AA)468
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