PCNA Protein (OPPA01753)

Catalog No: OPPA01753 (Formerly GWB-34BA50)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The PCNA solution contains 20mM Tris pH-7.5 & 2mM EDTA & 20% glycerol. Physical appearance: Sterile Filtered colorless solution.
• Host: E. Coli
  Product Introduction: PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps elevate the processivity of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and takes part in the RAD6-dependent DNA repair pathway. 2 transcript variants encoding the same protein have been found for PCNA gene. Pseudogenes of this PCNA gene have been described on chromosome 4 and on the X chromosome. PCNA is expressed during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA is induced by UV irradiation.
  Product Description: PCNA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 261 amino acids and having a molecular mass of 28769 Dalton.
• Reconstitution and Storage: PCNA although stable 4C for 4 weeks, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQLTLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S.

Target Info

• Gene Symbol: PCNA
• Alias Symbols: ATLD2
• NCBI Gene Id: 5111
• Protein Name: Proliferating cell nuclear antigen
• Description of Target: Recombinant Human Proliferating Cell Antigen
• Uniprot ID: P12004
• Protein Accession #: NP_002583.1
• Protein Size (# AA): Recombinant