Catalog No: OPPA01219 (Formerly GWB-52CE16)
Size:5UG
Price: $75.00
SKU
OPPA01219
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OPPA01219 |
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Predicted Species Reactivity | Human |
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Product Format | Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol. Physical appearance: Sterile Filtered colorless solution. |
Host | Sf9 Insect Cells |
Application | WB |
:: | Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera. |
:: | Product Introduction: Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis.Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10 % of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE. Product Description: PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 29,794 Dalton. PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles. |
Purity | Greater than 95% as determined by SDS-PAGE. |
Application Info | Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera. |
Gene Symbol | PCNA Sf9 |
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Alias Symbols | ATLD2 |
NCBI Gene Id | 5111 |
Protein Name | Proliferating cell nuclear antigen |
Description of Target | Recombinant Human Proliferating Cell Antigen, S9 |
Uniprot ID | P12004 |
Protein Accession # | NP_002583.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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