Catalog No: OKCA02133
Size:96 Wells
Price: $754.00
SKU
OKCA02133
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
Sample TypeDilutionMean (%)Range (%)
Serum (n=4)1:1009988-102
Serum (n=4)1:200112103-115
Serum (n=4)1:4009485-98
Serum (n=4)1:8009888-101
ELISA Kit PrincipleAviva Systems Biology PCNA ELISA Kit (Human) (OKCA02133) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PCNA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PCNA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PCNA captured in well.
ELISA Kit Range78 pg/mL-5,000 pg/mL
ELISA Kit Recovery
Sample TypeMean (%)Range (%)
Serum (n=5)111103-116
EDTA plasma (n=4)9688-101
ELISA Kit ReproducibilityMean Intra-Assay Variation <10% CV (n=20)
Mean Inter-Assay Variation <12% CV (n=8)
ELISA Kit Component
ComponentAmount
Anti-PCNA Microplate96 Wells (12 x 8 Well strips)
PCNA Lyophilized Standard 2 x 5 ng
100X Biotinylated PCNA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity19.5 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPCNA
Alias SymbolsATLD2
NCBI Gene Id5111
Protein NameProliferating cell nuclear antigen
Description of TargetAuxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Uniprot IDP12004
Protein Accession #NP_002583
Nucleotide Accession #NM_002592
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