PCNA Antibody (OAAD00193)

Catalog No: OAAD00193

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: IML-83
• Isotype: IgG2a
• Host: Mouse
• Application: WB, IHC, IP, ICC
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Protein A fusion protein
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 2ug/ml with the appropriate system to detect PCNA in cells and tissues.
  Immunohistochemistry(P) At 0.4-1ug/ml to detect PCNA in formalin fixed and paraffin embedded tissues. Boiling the sections may improve the staining.
  Immunohistochemistry(F) At 0.4-1ug/ml to detect PCNA in formalin or acetone fixed tissues.
  Immunocytochemistry Suitable Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Bravo, R. : Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replication.
  Exp. Cell Res. 163: 287-293, 1986.
  2. Moldovan, G.-L.; Pfander, B.; Jentsch, S. : PCNA controls establishment of sister chromatid cohesion during S phase. Molec. Cell 23: 723-732, 2006.
  3. Webb, G.; Parsons, P.; Chenevix-Trench, G. : Localization of the gene for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum. Genet. 86: 84-86, 1990.

Target Info

• Gene Symbol: PCNA
• Alias Symbols: ATLD2
• NCBI Gene Id: 5111
• Protein Name: Proliferating cell nuclear antigen
• Description of Target: Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA /cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4, 961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
• Uniprot ID: P12004