PCMT1 ELISA Kit (Human) (OKCD00734)

Catalog No: OKCD00734 (Formerly GWB-KBBGI4)

Size:96WELLS

Human PCMT1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-104%	85-96%	88-101%	95-105%
  EDTA plasma(n=5)	78-101%	92-104%	78-104%	80-101%
  heparin plasma(n=5)	79-95%	97-105%	93-104%	93-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein L-Isoaspartate-O-Methyltransferase (PCMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein L-Isoaspartate-O-Methyltransferase (PCMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein L-Isoaspartate-O-Methyltransferase (PCMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein L-Isoaspartate-O-Methyltransferase (PCMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	78-95	88
  EDTA plasma(n=5)	87-95	91
  heparin plasma(n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein L-Isoaspartate-O-Methyltransferase (PCMT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein L-Isoaspartate-O-Methyltransferase (PCMT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PCMT1 Microplate: 96 Wells (12 x 8 Well Strips)
  • PCMT1 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated PCMT1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 13.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein L-Isoaspartate-O-Methyltransferase (PCMT1). No significant cross-reactivity or interference between Protein L-Isoaspartate-O-Methyltransferase (PCMT1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PCMT1
• Gene Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Alias Symbols: epididymis secretory sperm binding protein;L-isoaspartyl protein carboxyl methyltransferase;PIMT;protein L-isoaspartyl/D-aspartyl methyltransferase;protein-beta-aspartate methyltransferase;protein-L-isoaspartate(D-aspartate) O-methyltransferase.
• NCBI Gene Id: 5110
• Protein Name: Protein-L-isoaspartate(D-aspartate) O-methyltransferase
• Description of Target: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
• Uniprot ID: P22061
• Protein Accession #: NP_001238978
• Nucleotide Accession #: NM_001252049.1
• Protein Size (# AA): 227