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Catalog No: OKCD07000
Size:96WELLS
Price: $625.00
SKU
OKCD07000
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Datasheets/ManualsPrintable datasheet for PCK1 ELISA Kit (Mouse) (OKCD07000)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PCK1 Microplate96 Wells (12 x 8 Well strips)
PCK1 Lyophilized Standard2
100X PCK1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.058ng/mL
Gene SymbolPck1
Gene Full Namephosphoenolpyruvate carboxykinase 1, cytosolic
Alias SymbolsAI265463;Pck-;Pck-1;PEP;PEPCK;PEPCK-C;phosphoenolpyruvate carboxykinase, cytosolic;phosphoenolpyruvate carboxykinase, cytosolic [GTP];phosphoenolpyruvate carboxylase;serine-protein kinase PCK1.
NCBI Gene Id18534
Protein NamePhosphoenolpyruvate carboxykinase, cytosolic [GTP]
Description of TargetCatalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Uniprot IDQ9Z2V4
Protein Accession #NP_035174.1
Nucleotide Accession #NM_011044.2
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