Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OCOA08965
Size:20UG
Price: $179.00
SKU
OCOA08965
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

PCDHB16 Protein Lysate (OCOA08965)

Datasheets/ManualsPrintable datasheet for PCDHB16 Protein Lysate (OCOA08965)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionPCDHB16 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolPCDHB16
Gene Full Nameprotocadherin beta 16
Alias Symbolscadherin ME1;ME1;PCDH3X;PCDHB8a;PCDH-BETA16;protocadherin beta 8a;protocadherin beta-16;protocadherin-3x.
NCBI Gene Id57717
Protein NameNM_020957, NP_066008, Q9NRJ7
Description of TargetHomo sapiens protocadherin beta 16 (PCDHB16), mRNA, Protocadherin beta-16, protocadherin beta-16 precursor [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q9NRJ7
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_066008
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_020957
Molecular Weight84.9 kDa
Write Your Own Review
You're reviewing:PCDHB16 Protein Lysate (OCOA08965)
Your Rating