PATCHED/PTCH1 Antibody (OABB01835)

Catalog No: OABB01835

Size:100ug

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Anti-Patched Picoband antibody, OABB01835, Western blotting
All lanes: Anti Patched (OABB01835) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 160KD
Observed bind size: 160KD

Product Info

• Tested Species Reactivity: Mouse, Rat
• Predicted Species Reactivity: Human
• Product Format: Lyophilized Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg sodium azide
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Immunogen: E.coli-derived human Patched recombinant protein (Position: D1219-N1447). Human Patched shares 91% amino acid (aa) sequence identity with mouse Patched.
• Purification: Immunogen affinity purified.
• Predicted Homology Based on Immunogen Sequence: Human
• Peptide Sequence: Synthetic peptide located within the following region: DSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN
• Specificity: No cross reactivity with other proteins.
• Application Info: WB: 0.5-1 ug/mL. Tested Species: Ms, Rat. Predicted Species: Hu. Antigen retrieval is by heat. The detection limit is approximately 0.1 ng/lane under reducing conditions.
• Reference: 1. A loss-of-function mutation in PTCH1 suggests a role for autocrine hedgehog signaling in colorectal tumorigenesis.Chung JH, et al. Oncotarget, 2013 Dec.
  2. Basal cell carcinosarcoma with PTCH1 mutations in both epithelial and sarcomatoid primary tumor components and in the sarcomatoid metastasis.Kiuru M, et al. Am J Surg Pathol, 2014 Jan.
  3. Proteomic analysis of PTCH1+/- fibroblast lysate and conditioned culture media isolated from the skin of healthy subjects and nevoid basal cell carcinoma syndrome patients.Ponti G, et al. Biomed Res Int, 2013.
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Protein patched homolog 1(PTCH1) detection. Tested with WB in Human;Mouse;Rat.

Target Info

• Gene Symbol: PTCH1
• Gene Full Name: patched 1
• Alias Symbols: A230106A15Rik, BCNS, FLJ26746, FLJ42602, Holoprosencephaly 7, HPE7, mes, NBCCS, OTTHUMP00000021709, OTTHUMP00000021710, Patched, Patched (Drosophila) homolog, Patched 1, Patched homolog (Drosophila), Patched homolog 1 (Drosophila), Patched homolog 1, Patched protein homolog 1, Protein patched homolog 1, PTC, PTC1, PTC1_HUMAN, PTCH, PTCH protein +12b, PTCH protein +4', PTCH protein -10, PTCH protein, PTCH1, PTCH1 protein, PTCH11, Ptch2
• NCBI Gene Id: 5727
• Protein Name: Protein patched homolog 1
• Description of Target: PTCH1 is also known as PTC, BCNS or HPE7. This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.
• Uniprot ID: Q13635