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Catalog No: OKEH00674
Size:96T
Price: $600.00
SKU
OKEH00674
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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PARP1 ELISA Kit (Human) : 96 Wells (OKEH00674)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PARP1 ELISA Kit (Human) (OKEH00674) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PARP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PARP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PARP1 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-PARP1 Microplate96 Wells (12 x 8 Well strips)
PARP1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PARP1 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.31 ng/mL
SpecificityNatural and recombinant Human Poly [ADP-ribose] polymerase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPARP1
Gene Full Namepoly(ADP-ribose) polymerase 1
Alias SymbolsADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase);ADP-ribosyltransferase diphtheria toxin-like 1;ADP-ribosyltransferase NAD(+);ADPRT;ADPRT 1;ADPRT1;ARTD1;DNA ADP-ribosyltransferase PARP1;NAD(+) ADP-ribosyltransferase 1;pADPRT-1;PARP;PARP-1;poly (ADP-ribose) polymerase family, member 1;poly [ADP-ribose] polymerase 1;poly(ADP-ribose) synthetase;poly(ADP-ribosyl)transferase;poly[ADP-ribose] synthase 1;PPOL;protein poly-ADP-ribosyltransferase PARP1.
NCBI Gene Id142
Protein NameP09874
Description of TargetPoly [ADP-ribose] polymerase 1
Uniprot IDhttps://www.uniprot.org/uniprot/P09874
Protein Accession #NP_001609.2
Nucleotide Accession #NM_001618.3
Protein Size (# AA)1014
Molecular Weight113 kDa
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