PARP1 Antibody (Phospho-Thr368) (OAAB16324)

Catalog No: OAAB16324 (Formerly GWB-U4R6CW)

Size:400ul

Dot blot analysis of Phospho-PARP1-T368 Antibody Phospho-specific Pab (Cat. # OAAB16324) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: DB
• Additional Information: Function: Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production
  Cellular Location: Nucleus. Nucleus, nucleolus. Note=Localizes at sites of DNA damage.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PARP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T368 of human PARP1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: DB: 1:500
• Reference: Majewski, P.M., et al. J. Biol. Chem. 285(45):34828-34838(2010)
  Kim, M., et al. Cancer Sci. 101(11):2436-2442(2010)
  Dong, Y., et al. Cancer Res. 70(20):8088-8096(2010)
  Krishnakumar, R., et al. Mol. Cell 39(5):736-749(2010)
  Lee, K.A., et al. Rheumatol. Int. (2010) In press :

Target Info

• Gene Symbol: PARP1
• Alias Symbols: PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1
• NCBI Gene Id: 142
• Protein Name: Poly [ADP-ribose] polymerase 1
• Description of Target: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
• Uniprot ID: P09874
• Protein Accession #: NP_001609.2
• Molecular Weight: 113 kDa