PAPPA2 ELISA Kit (Human) (OKCD00392)

Catalog No: OKCD00392 (Formerly GWB-KBBLR1)

Size:96WELLS

Human PAPPA2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	88-103%	80-91%	78-101%	79-101%
  EDTA plasma(n=5)	97-105%	80-91%	83-98%	84-93%
  heparin plasma(n=5)	80-103%	93-105%	79-103%	97-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pappalysin 2 (PAPPA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pappalysin 2 (PAPPA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pappalysin 2 (PAPPA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pappalysin 2 (PAPPA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	87-104	93
  EDTA plasma(n=5)	95-102	99
  heparin plasma(n=5)	97-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pappalysin 2 (PAPPA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pappalysin 2 (PAPPA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PAPPA2 Microplate: 96 Wells (12 x 8 Well Strips)
  • PAPPA2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated PAPPA2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.061 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pappalysin 2 (PAPPA2). No significant cross-reactivity or interference between Pappalysin 2 (PAPPA2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PAPPA2
• Gene Full Name: pappalysin 2
• Alias Symbols: PAPP-A2;pappalysin-2;PAPPE;PAPP-E;PLAC3;placenta-specific 3;pregnancy-associated plasma preproprotein-A2;Pregnancy-associated plasma protein A2;pregnancy-associated plasma protein E1;SSDA.
• NCBI Gene Id: 60676
• Protein Name: Pappalysin-2
• Description of Target: Metalloproteinase which specifically cleaves insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3.
• Uniprot ID: Q9BXP8
• Protein Accession #: NP_064714
• Nucleotide Accession #: NM_020318.2
• Protein Size (# AA): 1791