PAPPA ELISA Kit (Mouse) (OKCD06837)

Catalog No: OKCD06837

Size:96WELLS

PAPPA ELISA Kit (Mouse) (OKCD06837) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy Associated Plasma Protein A (PAPPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pregnancy Associated Plasma Protein A (PAPPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnancy Associated Plasma Protein A (PAPPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pregnancy Associated Plasma Protein A (PAPPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pregnancy Associated Plasma Protein A (PAPPA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pregnancy Associated Plasma Protein A (PAPPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PAPPA Microplate: 96 Wells (12 x 8 Well strips)
  • PAPPA Lyophilized Standard: 2
  • 100X PAPPA HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 29pg/mL

Target Info

• Gene Symbol: Pappa
• Gene Full Name: pregnancy-associated plasma protein A
• Alias Symbols: 8430414N03Rik;IGFBP-;IGFBP-4ase;IGF-dependent IGFBP-4 protease;insulin-like growth factor-dependent IGF-binding protein 4 protease;P;PA;PAG1;PAPP-A;pappalysin-1;Pregnancy-associated plasma protein A.
• NCBI Gene Id: 18491
• Protein Name: Pappalysin-1
• Description of Target: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Isoform 2 cleaves IGFBP-4 very slowly compared to PAPP-A, but its ability to cleave IGFBP-5 is unaffected.
• Uniprot ID: Q8R4K8
• Protein Accession #: NP_067337.1
• Nucleotide Accession #: NM_021362.1