PAPOLA ELISA Kit (Human) (OKWB00218)

Catalog No: OKWB00218

Size:96T

Human PAPOLA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-94%	86-97%	85-98%	87-103%
  EDTA Plasma (n=5)	82-89%	85-95%	83-96%	83-97%
  Heparin Plasma (n=5)	80-98%	88-100%	80-92%	82-94%

• ELISA Kit Principle: Aviva Systems Biology PAPOLA ELISA Kit (Human) (OKWB00218) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PAPOLA has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PAPOLA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PAPOLA captured in the well.
• ELISA Kit Range: 78.125-5000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-95	91
  EDTA Plasma (n=5)	86-105	98
  Heparin Plasma (n=5)	85-105	93

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • PAPOLA Microplate: 96 Wells (12 x 8 Well strips)
  • PAPOLA Lyophilized Standard: 2 x 5 ng
  • 100X PAPOLA Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 46.9 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PAPOLA
• Gene Full Name: poly(A) polymerase alpha
• Alias Symbols: PAP;PAP-alpha;poly(A) polymerase alpha;polynucleotide adenylyltransferase alpha.
• NCBI Gene Id: 10914
• Protein Name: Poly(A) polymerase alpha
• Description of Target: Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
• Uniprot ID: P51003
• Protein Accession #: NP_001238935
• Nucleotide Accession #: NM_001252006