PAOX ELISA Kit (Mouse) (OKCD01215)

Catalog No: OKCD01215 (Formerly GWB-KBBZN7)

Size:96WELLS

Mouse Paox ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	81-101%	97-104%	86-105%	93-101%
  EDTA plasma(n=5)	80-95%	90-98%	95-103%	84-94%
  heparin plasma(n=5)	91-101%	90-98%	98-105%	80-91%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polyamine Oxidase (PAOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polyamine Oxidase (PAOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polyamine Oxidase (PAOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Polyamine Oxidase (PAOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78.12-5000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-101	89
  EDTA plasma(n=5)	87-96	92
  heparin plasma(n=5)	96-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polyamine Oxidase (PAOX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polyamine Oxidase (PAOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Paox Microplate: 96 Wells (12 x 8 Well Strips)
  • Paox Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Paox Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 32 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Polyamine Oxidase (PAOX). No significant cross-reactivity or interference between Polyamine Oxidase (PAOX) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Paox
• Gene Full Name: polyamine oxidase (exo-N4-amino)
• Alias Symbols: 2410012F02Rik;AI118225;exo-N4-amino;Pao;peroxisomal N(1)-acetyl-spermine/spermidine oxidase;peroxisomal N1-acetyl-spermine/spermidine oxidase;Polyamine oxidase.
• NCBI Gene Id: 212503
• Protein Name: Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
• Description of Target: Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.
• Uniprot ID: Q8C0L6
• Protein Accession #: NP_722478
• Nucleotide Accession #: NM_153783.4
• Protein Size (# AA): 504