PALLD ELISA Kit (Human) (OKCD00284)

Catalog No: OKCD00284 (Formerly GWB-KBBDT8)

Size:96WELLS

Human PALLD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Palladin (PALLD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Palladin (PALLD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Palladin (PALLD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Palladin (PALLD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Palladin (PALLD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Palladin (PALLD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PALLD Microplate: 96 Wells (12 x 8 Well Strips)
  • PALLD Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated PALLD Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.051 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Palladin (PALLD). No significant cross-reactivity or interference between Palladin (PALLD) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PALLD
• Gene Full Name: palladin, cytoskeletal associated protein
• Alias Symbols: CGI151;CGI-151;MYN;myoneurin;palladin;PNCA1;sarcoma antigen NY-SAR-77;SIH002.
• NCBI Gene Id: 23022
• Protein Name: Palladin
• Description of Target: Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.
• Uniprot ID: Q8WX93
• Protein Accession #: NP_001159580
• Nucleotide Accession #: NM_001166108.1
• Protein Size (# AA): 1383