PAK4 Antibody - N-terminal region (OAAB17318)

Catalog No: OAAB17318

Size:400ul

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PAK4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC
• Additional Information: Precautions: PAK4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN
  Cellular Location: Cytoplasm. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways
  Tissue Location: Highest expression in prostate, testis and colon
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PAK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-187 amino acids from the N-terminal region of human PAK4.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IHC-P: 1:10~50
  WB: 1:1000

Target Info

• Gene Symbol: PAK4
• Alias Symbols: PAK4, KIAA1142, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4
• NCBI Gene Id: 10298
• Protein Name: Serine/threonine-protein kinase PAK 4
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O96013
• Protein Accession #: NP_001014831.1
• Molecular Weight: 64072 Da