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Datasheets/Manuals | Printable datasheet for PAK2 Antibody (Phospho-Ser141) (OAAF00467) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human PAK2 around the phosphorylation site of Ser141. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | PAK2 (Phospho-Ser141) antibody detects endogenous levels of PAK2 only when phosphorylated at serine 141. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; ELISA: 1:10000 |
Reference | Sells M., Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases. The MGC Project Team; Genome Res. 14:2121-2127(2004). Martin G.A., EMBO J. 14:1970-1978(1995). |
Gene Symbol | PAK2 |
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Gene Full Name | p21 (RAC1) activated kinase 2 |
Alias Symbols | gamma-PAK;p21 (CDKN1A)-activated kinase 2;p21 protein (Cdc42/Rac)-activated kinase 2;p21-activated kinase 2;p58;PAK-2;PAK65;PAKgamma;S6/H4 kinase;serine/threonine-protein kinase PAK 2. |
NCBI Gene Id | 5062 |
Protein Name | Serine/threonine-protein kinase PAK 2 |
Description of Target | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation. |
Uniprot ID | Q13177 |
Protein Accession # | NP_002568.2 |
Molecular Weight | 58 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PAK2 Antibody (Phospho-Ser141) (OAAF00467)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "PAK2 Antibody (Phospho-Ser141) (OAAF00467)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "PAK2 Antibody (Phospho-Ser141) (OAAF00467)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PAK2 Antibody (Phospho-Ser141) (OAAF00467)"?
This target may also be called "gamma-PAK;p21 (CDKN1A)-activated kinase 2;p21 protein (Cdc42/Rac)-activated kinase 2;p21-activated kinase 2;p58;PAK-2;PAK65;PAKgamma;S6/H4 kinase;serine/threonine-protein kinase PAK 2." in publications.
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What is the shipping cost for "PAK2 Antibody (Phospho-Ser141) (OAAF00467)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PAK2 Antibody (Phospho-Ser141) (OAAF00467)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PAK2 Antibody (Phospho-Ser141) (OAAF00467)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "58 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PAK2 Antibody (Phospho-Ser141) (OAAF00467)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PAK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PAK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PAK2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PAK2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PAK2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PAK2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.