PAK1 Antibody (Phospho-Thr212) (OAAB16169)

Catalog No: OAAB16169

Size:400ul

Dot blot analysis of Phospho-PAK1-T212 Pab (Cat.OAAB16169) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: DB
• Additional Information: Precautions: Phospho-PAK1-T212 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2
  Cellular Location: Cytoplasm. Cell junction, focal adhesion. Cell membrane. Note=Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PAK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T212 of human PAK1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: DB: 1:500

Target Info

• Gene Symbol: PAK1
• Alias Symbols: IDDMSSD, p65-PAK, PAKalpha, alpha-PAK
• NCBI Gene Id: 5058
• Protein Name: Serine/threonine-protein kinase PAK 1
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q13153
• Protein Accession #: NP_001122092.1
• Molecular Weight: 60647 Da