PAH ELISA Kit (Mouse) (OKCA01742)

Catalog No: OKCA01742

Size:96 Wells

Mouse PAH ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology Pah ELISA Kit (Mouse) (OKCA01742) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pah has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pah is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Pah captured in the well.
• ELISA Kit Range: 23.5 - 1,500 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	86	83-89
  EDTA Plasma (n=4)	89	85-96

• ELISA Kit Component:
  • Pah Microplate: 96 Wells (12 x 8 Well strips)
  • Pah Lyophilized Standard: 2 x 1.5 ng
  • 100X Biotinylated Pah Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates and cell lysates
• Sensitivity: 5.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: PAH
• Gene Full Name: phenylalanine hydroxylase
• Alias Symbols: AW106920
• NCBI Gene Id: 18478
• Protein Name: Phenylalanine-4-hydroxylase
• Description of Target: Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
• Uniprot ID: P16331
• Protein Accession #: NP_032803.2
• Nucleotide Accession #: NM_008777.3