PAG1 Antibody (OAEE00513)

Catalog No: OAEE00513

Size:100UG

Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255).
Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-255
• Isotype: IgG2a
• Host: Mouse
• Application: Flow cytometry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Svec A, Velenská Z, Horejsí V: Expression pattern of adaptor protein PAG: correlation between secondary lymphatic follicle and histogenetically related malignant lymphomas. Immunol Lett. 2005 Aug 15;100(1):94-7.
  Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers.Blood. 2006 Jan 1;107(1):213-21. Epub 2005 Sep 13.
  Davidson D, Bakinowski M, Thomas ML, Horejsi V, Veillette A: Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor.Mol Cell Biol. 2003 Mar;23(6):2017-28. , Semac I, Palomba C, Kulangara K, Klages N, van Echten-Deckert G, Borisch B, Hoessli DC: Anti-CD20 therapeutic antibody rituximab modifies the functional organization of rafts/microdomains of B lymphoma cells. Cancer Res. 2003 Jan 15;63(2):534-40.
  Brdickova N, Brdicka T, Andera L, Spicka J, Angelisova P, Milgram SL, Horejsi V: Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett. 2001 Oct 26;507(2):133-6.
  Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), anovel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.
  Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.

Target Info

• Gene Symbol: PAG1
• Gene Full Name: phosphoprotein membrane anchor with glycosphingolipid microdomains 1
• Alias Symbols: CBP;Csk-binding protein;PAG;phosphoprotein associated with glycosphingolipid microdomains 1;phosphoprotein associated with glycosphingolipid-enriched microdomains 1;transmembrane adapter protein PAG;transmembrane adaptor protein PAG;transmembrane phosphoprotein Cbp.
• NCBI Gene Id: 55824
• Protein Name: Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• Description of Target: Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
• Uniprot ID: Q9NWQ8
• Protein Accession #: NP_060910.3
• Nucleotide Accession #: NM_018440.3