PAFAH2 ELISA Kit (Human) (OKCD07911)

Catalog No: OKCD07911

Size:96WELLS

PAFAH2 ELISA Kit (Human) (OKCD07911) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Activating Factor Acetylhydrolase 2 (PAFAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PAFAH2 Microplate: 96 Wells (12 x 8 Well strips)
  • PAFAH2 Lyophilized Standard: 2
  • 100X PAFAH2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma.
• Sensitivity: < 0.105ng/mL

Target Info

• Gene Symbol: PAFAH2
• Gene Full Name: platelet activating factor acetylhydrolase 2
• Alias Symbols: HSD-PLA2;PAF:lysophospholipid transacetylase;PAF:sphingosine transacetylase;platelet-activating factor acetylhydrolase 2, 40kDa;platelet-activating factor acetylhydrolase 2, cytoplasmic;platelet-activating factor acetyltransferase PAFAH2;SD-PLA2;serine-dependent phospholipase A2.
• NCBI Gene Id: 5051
• Protein Name: Platelet-activating factor acetylhydrolase 2, cytoplasmic
• Description of Target: Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme.
• Uniprot ID: Q99487
• Protein Accession #: NP_000428.2
• Nucleotide Accession #: NM_000437.3