PAFAH1B1 Antibody - N-terminal region (ARP56108_P050)

Catalog No: ARP56108_P050

WB Suggested Anti-PAFAH1B1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Lung

Rabbit Anti-PAFAH1B1 Antibody
Catalog Number: ARP56108_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic in endothelial cells in blood vessels
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PAFAH1B1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%
• Peptide Sequence: Synthetic peptide located within the following region: KLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVF
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PAFAH1B1 (ARP56108_P050) antibody is Catalog # AAP56108 (Previous Catalog # AAPP38013)
• Subunit: alpha
• Reference: Campo,S., (2008) Aging Clin Exp Res 20 (2), 171-177

Target Info

• Gene Symbol: PAFAH1B1
• Gene Full Name: Platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
• Alias Symbols: MDS, LIS1, LIS2, MDCR, NudF, PAFAH
• NCBI Gene Id: 5048
• Protein Name: Platelet-activating factor acetylhydrolase IB subunit alpha
• Description of Target: This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 is the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.PAFAH1B1 was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-378 AC015799.23 48129-48506 c 379-600 AC015799.23 3815-4036 c 601-685 AC005696.1 40697-40781 686-760 AC005696.1 41341-41415 761-967 AC005696.1 42317-42523 968-1136 AC005696.1 45488-45656 1137-1239 AC005696.1 47980-48082 1240-1468 AC005696.1 49385-49613 1469-1570 AC005696.1 51830-51931 1571-1727 AC005696.1 55489-55645 1728-5614 AC005696.1 57054-60940
• Uniprot ID: P43034
• Protein Accession #: NP_000421
• Nucleotide Accession #: NM_000430
• Protein Size (# AA): 410
• Molecular Weight: 47kDa
• Protein Interactions: UBI4; UBC; SUMO2; BICD2; SUMO1; PDIA4; PAPSS1; YWHAH; YWHAG; SNRPF; SNRPD2; PAK2; MYO1E; MSN; HSP90AA4P; HMGB3; FH; EIF5; CAPNS1; CAPN2; ATIC; ASNS; TATDN1; UBXN1; ISOC1; MAPRE1; NDE1; IQCB1; SLC25A10; DCX; NUDCD2; NDEL1; tat; KATNA1; NUDC; KATNB1; CLIP1;