PADI4 Recombinant Protein (OPCD05952)

Catalog No: OPCD05952

PADI4 Recombinant Protein (OPCD05952) using SDS-PAGE

PADI4 Recombinant Protein (OPCD05952) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Met1-Pro300
  Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic granule.
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 95%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: PADI4
• Gene Full Name: peptidyl arginine deiminase 4
• Alias Symbols: HL-60 PAD;PAD;PAD4;PADI5;PADI-H protein;PDI4;PDI5;peptidyl arginine deiminase, type IV;peptidyl arginine deiminase, type V;Peptidylarginine deiminase IV;protein-arginine deiminase type IV;protein-arginine deiminase type-4.
• NCBI Gene Id: 23569
• Protein Name: Protein-arginine deiminase type-4
• Description of Target: Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
• Uniprot ID: Q9UM07
• Protein Accession #: NP_036519.2
• Nucleotide Accession #: NM_012387.2
• Protein Size (# AA): Met1~Pro300
• Molecular Weight: 36kDa