Catalog No: OKCA00921
Size:96 Wells
Price: $754.00
SKU
OKCA00921
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9185-99
1:29589-101
1:49692-110
1:89386-102
ELISA Kit PrincipleAviva Systems Biology Padi4 ELISA Kit (Mouse) (OKCA00921) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Padi4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Padi4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Padi4 captured in well.
ELISA Kit Range4.7 pg/mL-300 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9788-105
EDTA Plasma (n=4)9689-104
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Padi4 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Padi4 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
Padi4 Microplate96 Wells (12 x 8 Well strips)
Padi4 Lyophilized Standard2
100X Biotinylated Padi4 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity1.17 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPADI4
Gene Full NamePeptidyl arginine deiminase, type IV
Alias SymbolsPa, Pd, Pad4, Pdi4
NCBI Gene Id18602
Protein NameProtein-arginine deiminase type-4
Description of TargetCatalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Uniprot IDQ9Z183
Protein Accession #NP_035191.2
Nucleotide Accession #NM_011061.2
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