Catalog No: OKCD01879 (Formerly GWB-KBBTK1)
Size:96WELLS
Price: $675.00
SKU
OKCD01879
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Datasheets/ManualsPrintable datasheet for PADI4 ELISA Kit (Human) (OKCD01879)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)97-104%85-94%83-96%90-104%
EDTA Plasma (n=5)85-102%78-98%98-105%91-99%
Heparin Plasma (n=5)98-105%88-103%78-97%78-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type IV (PADI4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type IV (PADI4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type IV (PADI4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidyl Arginine Deiminase Type IV (PADI4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)82-9489
EDTA Plasma (n=5)78-9489
Heparin Plasma (n=5)86-9591
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type IV (PADI4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type IV (PADI4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PADI4 Microplate96 Wells (12 x 8 Well Strips)
PADI4 Lyophilized Standard2 x 20 ng
100X Biotinylated PADI4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.121 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type IV (PADI4). No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type IV (PADI4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPADI4
Gene Full Namepeptidyl arginine deiminase 4
Alias SymbolsHL-60 PAD;PAD;PAD4;PADI5;PADI-H protein;PDI4;PDI5;peptidyl arginine deiminase, type IV;peptidyl arginine deiminase, type V;Peptidylarginine deiminase IV;protein-arginine deiminase type IV;protein-arginine deiminase type-4.
NCBI Gene Id23569
Protein NameProtein-arginine deiminase type-4
Description of TargetCatalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Uniprot IDQ9UM07
Protein Accession #NP_036519.2
Nucleotide Accession #NM_012387.2.
Protein Size (# AA)663
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