PABPC4 Antibody - N-terminal region (ARP40496_T100)

Catalog No: ARP40496_T100

WB Suggested Anti-PABPC4 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

Rabbit Anti-PABPC4 Antibody
Catalog Number: ARP40496
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PABPC4
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 93%; Rat: 100%; Zebrafish: 79%
• Peptide Sequence: Synthetic peptide located within the following region: AELGAKAKEFTNVYIKNFGEEVDDESLKELFSQFGKTLSVKVMRDPNGKS
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-PABPC4 (ARP40496_T100) antibody is Catalog # AAP40496 (Previous Catalog # AAPP22264)
• Reference: Lehner,B. (2004) Genome Res. 14 (7), 1315-1323

Target Info

• Gene Symbol: PABPC4
• Gene Full Name: Poly(A) binding protein, cytoplasmic 4 (inducible form)
• Alias Symbols: APP1, APP-1, PABP4, iPABP
• NCBI Gene Id: 8761
• Protein Name: Polyadenylate-binding protein 4
• Description of Target: Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.
• Uniprot ID: Q13310
• Protein Accession #: NP_003810
• Nucleotide Accession #: NM_003819
• Protein Size (# AA): 644
• Molecular Weight: 71kDa
• Protein Interactions: FBXW11; UBC; IVNS1ABP; STAU1; RPA3; RPA2; RPA1; WWOX; SUZ12; RNF2; EZH2; BMI1; NCAPD2; DDX5; TARDBP; SRPK2; CLK3; YWHAQ; UBD; EIF2AK2; PAN2; UBL4A; SMAD9; ITGA4; IL7R; IFIT3; IFIT1; IFIT2; FN1; IGBP1; NUBP2; CAND1; DCUN1D1; COPS5; CUL1; CUL2; CUL3; CUL4A;