P53 ELISA Kit (Human) (OKBB00357)

Catalog No: OKBB00357

Size:96 Tests

Human TP53 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human P53 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P53 has been precoated onto 96-well plates. Standards(NS0, M1-D393) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P53 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human P53 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	1328	2789	5434	1502	2891	6120
  St. Dev.	78.4	173	353.21	103.7	214	508
  %CV	5.90	6.20	6.5	6.90	7.40	8.30

• ELISA Kit Component:
  • Lyophilized recombinant human P53 standard: 10 ng/tube x 2
  • Anti-human P53 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human P53 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 156pg/ml-10,000pg/ml
  Principle: Aviva's human P53 ELISA Kit was based on standard sandwich
  enzyme-linked immune-sorbent assay technology. Human P53 specific-specific
  polyclonal antibodies were precoated onto 96-well plates. The human specific
  detection polyclonal antibodies were biotinylated. The test samples and
  biotinylated detection antibodies were added to the wells subsequently and
  then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase
  Complex was added and unbound conjugates were washed away with PBS or
  TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction.
  TMB was catalyzed by HRP to produce a blue color product that changed into
  yellow after adding acidic stop solution. The density of yellow is proportional to
  the human P53 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don't let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don't reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell lysates
• Sensitivity: <10 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Matlashewski G, Lamb P, Pim D, Peacock J, Crawford L, Benchimol S (December 1984). "Isolation and characterization
  of a human p53 cDNA clone: expression of the human p53 gene". EMBO J. 3 (13): 3257–62. PMC 557846. PMID
  6396087.
  2. Isobe M, Emanuel BS, Givol D, Oren M, Croce CM (1986). "Localization of gene for human p53 tumour antigen to band
  17p13". Nature 320 (6057): 84–5. doi:10.1038/320084a0. PMID 3456488.
  3. Kern SE, Kinzler KW, Bruskin A, Jarosz D, Friedman P, Prives C, Vogelstein B (June 1991). "Identification of p53 as a
  sequence-specific DNA-binding protein". Science (journal) 252 (5013): 1708–11. doi:10.1126/science.2047879. PMID
  2047879.
  4. McBride OW, Merry D, Givol D (1986). "The gene for human p53 cellular tumor antigen is located on chromosome 17
  short arm (17p13)". Proc. Natl. Acad. Sci. U.S.A. 83 (1): 130–134. doi:10.1073/pnas.83.1.130. PMC 322805. PMID
  3001719.
• Specificity: Natural and recombinant human P53
• Immunogen: Expression system for standard: NS0; Immunogen sequence: M1-D393
• Description: For quantitative detection of human P53 in cell lysates.

Target Info

• Gene Symbol: TP53
• Gene Full Name: tumor protein p53
• Alias Symbols: Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, LFS1, MS980, p53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor suppressor p53
• NCBI Gene Id: 7157
• Protein Name: Cellular tumor antigen p53
• Description of Target: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.
• Uniprot ID: P04637
• Protein Accession #: NP_000537.3
• Nucleotide Accession #: NM_000546.5