P53 Antibody (Phospho-Ser20) (OAEC00331)

Catalog No: OAEC00331

Size:50ug

Western blot analysis of extracts from COS7 cells treated with UV. Left: using P53 (phospho-Ser20) antibody (OAEC00331). Right: using the same antibody preincubated with synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P53 (phospho- Ser20) antibody (OAEC00331).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, ELISA
• Reconstitution and Storage: Store at -20°C/1 year
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human P53 around the phosphorylation site of serine 20 (T-F-S^P-D-L).
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Concentration: 1 ug/ul
• Specificity: P53 (phospho-Ser20) antibody detects endogenous levels of P53 only when phosphorylated at serine 20.
• Application Info: WB: 1:500~1000
  IHC: 1:50~100
  ELISA: 1:4000
  Optimal dilutions should be determined by the end user.

Target Info

• Reference: Wang H, et al. (2008) J Biol Chem 283, 2564-74
  Mayo LD, et al. (2005) J Biol Chem 280, 25953-9
  Thompson T, et al. (2004) J Biol Chem 279, 53015-22
  Hirao, A. et al. (2000) Science 287, 1824-1827
  Levine, A.J. (1997) Cell 88, 323-331
  Shieh, S.Y. et al. (1997) Cell 91, 325-334
• Gene Symbol: TP53
• Gene Full Name: Cellular tumor antigen p53
• Alias Symbols: P53, BCC7, LFS1, BMFS5, TRP53
• NCBI Gene Id: 7157
• Protein Name: Cellular tumor antigen p53
• Uniprot ID: P04637
• Protein Accession #: NP_000537.3